SPIFE Proteins

Split-Beta or 5-Band SPE

While some laboratorians prefer classic serum protein separations, others prefer a split-beta separation. With SPIFE Split-Beta gels, the proteins migrating in the beta region are split into two fractions, Beta 1 and Beta 2, between transferrin and C3 complement. In some instances, this may allow easier detection of small beta-migrating monoclonal gammopathies. Both the classic and split-beta formulations provide crisp, clear separation of the protein fractions. Light Chain Disease.

SPIFE Protein SPE 20

●	Samples are applied neat, no dilution hassles
●	SPIFE’s application system is unaffected by triglycerides or large proteins
●	Throughput of 1,400 samples in one 8-hour shift
●	New QuickGel 10/20 sample gels plus SPIFE 20, 40, 60, 80 &
	100 sample gels to suit any testing volume

Classic Serum Protein Kits
Split Beta Serum Protein Kits
QuickGel Split Beta SPE Kit

Classic Serum Protein Kits

Cat. No.	Item	Description
3460	SPIFE SPE-60 Kit (600 Profiles)	10 x 60 samples
3461	SPIFE SPE-40 Kit (400 Profiles)	10 x 40 samples
3462	SPIFE SPE-20 Kit (200 Profiles)	10 x 20 samples

Kits include: 10 Gels, Acid Blue Stain, Citric Acid Destain, Blotters, Modified Applicator Blades.

Classic Urine and CSF Protein Kits

Cat. No.	Item	Description
3460U	SPIFE SPE-60 Kit for Urines	10 x 60 samples
3461U	SPIFE SPE-40 Kit for Urines	10 x 40 samples
3462U	SPIFE SPE-20 Kit for Urines	10 x 20 samples

Kits include: 10 Gels, Acid Blue Stain, Citric Acid Destain, Blotters, Modified Applicator Blades.

Split Beta Serum Protein Kits

Cat. No.	Item	Description
3398	SPIFE Split Beta SPE-100 Kit	10 x 100 samples
3399	SPIFE Split Beta SPE-80 Kit	10 x 80 samples
3420	SPIFE Split Beta SPE-60 Kit	10 x 60 samples
3421	SPIFE Split Beta SPE-40 Kit	10 x 40 samples
3421T	SPIFE Split Beta SPE-40 Kit (Template Application)	10 x 40 samples
3422	SPIFE Split Beta SPE-20 Kit	10 x 20 samples

Kits include: 10 Gels, Acid Blue Stain, Citric Acid Destain, Blotters, Modified Applicator Blades.

Split Beta Urine and CSF Protein Kits

Cat. No.	Item	Description
3398U	SPIFE Split Beta SPE-100 Kit for Urines	10 x 100 samples
3399U	SPIFE Split Beta SPE-80 Kit for Urines	10 x 80 samples
3420U	SPIFE Split Beta SPE-60 Kit for Urines	10 x 60 samples
3421U	SPIFE Split Beta SPE-40 Kit for Urines	10 x 40 samples
3422U	SPIFE Split Beta SPE-20 Kit for Urines	10 x 20 samples

Kits include: 10 Gels, Acid Blue Stain, Citric Acid Destain, Blotters, SPIFE Applicator Blades.

Additional Supplies and Equipment for QuickGel Split Beta and SPE SPIFE Procedures: Applicator Blade Weights (Cat. No. 3387), QuickGel Gel Block Remover (Cat. No. 1262), SPE Normal (Cat. No. 3424), SPE Abnormal (Cat. No. 3425), REP Prep (Cat. No. 3100), QuickGel Dispo Cup Tray (Cat. No. 3353), SPIFE QuickGel Electrode (Cat. No. 1111), SPIFE QuickGel Holder (Cat. No. 3358), SPIFE QuickGel Chamber Alignment Guide (Part No. 86541003)

QuickGel Split Beta Kit for SPIFE

Cat. No.	Item	Description
3350	QuickGel Split Beta SPE Kit	10 x 10/20 samples*
3405	QuickGel SPE Kit for SPIFE (5-Band)	10 x 10/20 samples*

Kits include: 10 QuickGel Gels, Acid Blue Stain, QuickGel Blotters, Citric Acid Destain, 10 Modified Applicator Blades for use with serum samples.
* Additional Blades (Cat No 3451) and Sample Cups (Cat No 3460) can be purchased to run up to 20 serum samples per gel. For CSF and urines, SPIFE Applicator Blades (Cat No 3450) and Shallow Well Sample Cups (Cat No 3369) must be used or the samples applied using a QuickGel template (Cat No3426).

Serum Protein Controls

Cat. No.	Item
3424	SPE Normal Control
3425	SPE Abnormal Control

These lyophilized controls are prepared from pooled human serum and are for use with the SPIFE SPE Vis and Split-Beta SPE kits listed above. Reconstituted controls are stable 5 days stored at 2 to 8° C. Each control is assayed for total protein, albumin, alpha1, alpha2, beta and gamma globulins.

Applicator Blades, Cups and Templates

For Serum samples use
3451	SPIFE Modified Applicator Blades, 30/pkg
3360	SPIFE Dispo Sample Cups - Deep Well, 100/pkg

For Urine and CSF samples use
3450	SPIFE Applicator Blades, 30/pkg
3369	SPIFE Dispo Sample Cups - Shallow Well, 100/pkg

NOTE: Urine and CSF specimens with insufficient volumes for automated sample application may be run using template application:

For SPIFE Classic and Split Beta SPIFE Kits use
3427	SPIFE Urine/CSF Protein Accessory Kit (50 templates and blotters)
3380	SPIFE Urine IFE Alignment Tray

For QuickGel Split Beta and SPE Kits use
3426	QuickGel Accessory Kit (10 templates and blotters)

SPIFE 3000 Assays

SPIFE 3000

The SPIFE 3000 separates and develops up to 1400 SPE samples or 210 IFE profiles in an 8-hour shift, with integrated sample and reagent application. Its barcode "lockout" feature provides positive ID between sample tray and electrophoresis gel.